MMB Norovirus real-time PCR Kit from MilliporeSigma


The comprehensive MMB Food Pathogen System consists of matched kits, combining those for the isolation of nucleic acids (MMB Food Pathogen Prep Kits) with kits for real-time PCR-based detection of food samples (MMB Food Pathogen Detection Kits).

All tests follow the same simple procedure of enrichment/concentration, sample preparation and real-time PCR detection. Fail-safe analysis is completed within a few hours of enrichment, ensuring a short time to result for fast product release. Identical amplification protocols for all microorganisms allow different tests to be performed in a single run. Simultaneous amplification of the desired target DNA and of an internal control DNA excludes false negative results. The use of a Hot-Start enzyme for amplification further increases sensitivity.

The real-time PCR method using MMB Norovirus Kits enables the rapid and sensitive testing of raw material and processed food for absence of Norovirus. The test procedure can be performed using all common real-time cyclers. Distinct versions of the test kit are available for different instrument types: All MMB Norovirus Kits contain FAM-labeled probes for the detection of Norovirus RNA at 522 nm. The MMB Norovirus (V) Kit uses the fluorescent dye VIC for the detection of the internal amplification control, MMB Norovirus (R) Kit uses dye ROX and MMB Norovirus (LC) Kit dye XL-dye. For use with other detection platforms different variants are available, containing fluorescent labels which are detected on other channels:

  • MMB Norovirus (V) Kit: VIC channel (553 nm)
  • MMB Norovirus (R) Kit: ROX channel (602 nm)
  • MMB Norovirus (LC) Kit: LightCycler detection (640 nm)

For isolation of Norovirus RNA the MMB DNA/RNA Virus Prep Kit is recommended.

The MMB Food Pathogen System allows fast and sensitive detection of foodborne bacteria, including Bacillus cereus, Clostridium perfringens, Legionella, Staphylococcus aureus and Vibrio cholerae.

For the detection of Brucella, Campylobacter, E. coli O157, Enterobacteriaceae & E. sakazakii, Listeria, Salmonella, Shigella, beer spoilage bacteria, Saccharomyces cerevisiae var. diastaticus, Dekkera (Brettanomyces) and Alicyclobacillus please refer to our foodproof® real-time PCR portfolio which completes our range of pathogen testing solutions.

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